백서에서 Glucocorticoids 투여시 골결손부 치유에 미치는 영향에 관한 연구

Study about the Effect of Glucocorticoids on the Healing Process at the Bone Defect in Rat

The main purpose of this study was to examine histologically the effects of the Glucocorticoids in the site of Bone defect during the Healing Process of the Bone in Rat. A total of 36 adults Sprague-Dawley malerats, weighing 200gm were used in this experiment group. The others were used as a cintrol group and the rest, 4 rats were sacrificed to measure the Alkaline Phosphatase. Bone defect was performed into the left proximal tibia 2mm beneath from the knee joint deliberately and they were sacrified 3 days, 1 week, 2 weeks and 4 weeks after operation and glucocortucoids were injected into the experimental group(16 rats) as a manner of 18mg/day following 7 days of operation and the other group(16 rats) was not medicated. After completed above procedures, specimen were fixed into 10% neutral formalin. Specimens were examined under light microscope with Hematoxylin-Eosin and Masson s Trichrome stain.\ From the above results were as follows: 1.In the H&E stain control group, the inflammatory cells were infiltrated with partially calcified bone at the 3rd days minimally and cartilages were appeared and bone resorption and calcifications were progressed continuously within the 1st week. At the 4th week, the bone formation and irregular calcifications were examined. 2.In the experimental group on H&E stain, more inflammatory cell infiltrated by the 3rd dats and the new bone was observed on the 1st week and ossification of the cartilagenous tissue, formation of bone marrow and calcification was more progrssed. 3.In MT stain of control group, cartilage formation was extensive in the 3rd and 1st week. Bone trabeculae were accumulated at the 2nd week and bone remodeling was seen at the 4th week. 4.In MT stain of experimental group, cartilage and new bone formation was seen in the 3rd days. Cartilagenous tissue was replaced by the bone on the 2nd weeks and accumulation of osteoids were observed. 5.Alkaline phosphatase was increased up to the 2nd week and prominently decreased in the 4th week at the control group. However, there was a tendency to decrease in the 1st week and increase after 4 week remarkably. From the above results, it was recognized that glucocorticoids were profoundly influenced on bone remodeling in experimental group, decreased the number of osteocytes and decreased accumulation of lacunae.