우골에서 추출한 골형성단백을 이용한 백서 두개골 결손부 치유에 관한 연구

A HISTOLOGIC STUDY ON THE REPAIR OF RAT CALVARIAL CRITICAL SIZE DEFECT WITH BOVINE BONE MORPHOGENETIC PROTEIN (b-BMP)

Bovine BMP had been partially purified by guanidine HCI extraction and heparin affinity chromatography. It had distinct 3 bands between 20kDa and 30kDa in SDS-polyacrylamide gel electrophoresis. The critical size defect of rat calvaria, 8mm size in diameter, was used to establish osteoinductive activity of BMP and other bone graft materials. Bovine BMP plus IBM(inactivated bone matrix) as a carrier, allogenic demineralized freeze-dried bone (DFDB) and autogenous cortico-cancellous bone were implanted into the 12 rat skull defect of each group in order to evaluate repairing capacity The animals were sacrificed at the week of 2nd, 4th, 6th and 8th after operation The results were as follows 1. In the BMP-IBM complex Implantation group, the defect has been repaired completely by the 8th postoperative week. At 2 week, duster of chondroblast and cartilage appeared around the BMP-coated implant matrix. Calcification began at 6 week. At 8 week, the woven bone changed lamella bone with hematopoietic marrow and it has been bridged the both end of skull defect. 2. The repairing speed and quantity of new bone formation of BMP-IBM complex implant were superior to those of autogenous bone graft and DFDB implant. The defect which was filled with DFDB healed not only by new bone but also by retained implanted bone. The inflammatory and immune reaction of DFDB graft were more apparent than those of BMP implant and autogenous bone graft. 3. It has been assumed that the bovine BMP could be effectively used for the treatment of complicated bone defects in oral and maxillofacial region.