연골성 및 막성 탈회골기질과 탈회상아질기질에 의한 골유도 과정에 대한 연구

Bone formation processes induced by demineralized endochondral and intramembranous bone matrix and dentin matrix

Bony tissue develops via two different preocesses : endochondral ossification and intramembanous ossification. This study tests the hypothesis that each type of bony tissue contains unique inducing factors for the promotion of cartilage and direct bone formation. Previous studies suggest that both of implants of intramembranous and endchondral bone matrix can induce bone formation through endochondral bone formation, which means that the bone growth properties of the bone matrices are similar. This study was done to know whether the osteoinductive property of intramembranous bone matrix is identical to that of endochondral matrix and that property of demineralized dentin matrix. Endochondral and intramembranous demineralized bone matrices and demineralized dentin matrices were implanted in pectoral muscles of rats. At the 5 days of implantation many spindle shaped mesenchymal cells aggregated around 3 kinds of the matrices. At the 10 days of implantation osteoid tissue formed adjacent to the matrices. At the 14 days implants of endochondral bone and dentin matrices induced bone formation via endochodral and intramembranous ossification in contrast to implsants of intramembranous bone matrix which did not induce endochondral ossification. At the 60 days of implantation, the matrices were absorbed and mature bone formation with trabeculae was proceeded. The matrices were aborbed by macrophages and multinucleated giant cells and osteoclasts which resorb the calcified bone at the same time. These findings suggest that endochondral ossification is not a necessity in the processes of bone formation by demineralized hard tissue matrix and the matrix of endochondral bone differs qualitatively from that of intramembranous bone.