가토 두개골 결손부에서 막성 및 연골성 탈회골기질의 재생능에 관한 연구

A study on the regenerative capacity of endochondral and membranous demineralized allogenic bone matrix graft in rabbit

This study was done to examine the effect of demineralized allogenic bone matrix graft of membranous and endochondral origin compared with autogenous bone chips on healing response in rabbit skull defects. Four calvarial defects were made and demineralized allogenic bone matrix powder membranous and endochondral origin and autogenous bone chips were implanted in each of three defects ad leaving the fourth defect empty for control. Undifferentiated mesenchymal cells appeared in autogenous bone and both demineralized allogenic bone graft groups, but inflammatory cell infiltration appeared in control group at 1 week after graft. Two weeks after graft, new bone formation appeared in al grafted area except control defect and the most active bone formation was observed in autogenous bone graft group and the least appeared in demineralized allogenic bone of membranous origin. Four weeks after graft, profuse bone formation appeared in entire surface of autogenous bone grafted area and trabecular foramtion appeared in both demineralized allogenic bone graft group, but the degree of trabeculation was smaller in membranous origin than in endochondral origin. Active bone formation was observed in control defect at 4 weeks after graft. Six weeks after graft, mature bone formation was seen at grafted area except control group. Complete trabeculation was observed in all experimental groups at 8 weeks after graft.