골형성단백질(BMP)의 골유도 실험에서 맥동전자기장의 효과에 관한 연구

The experimental study on the effect of pulsating electromagnetic fields in te osteoinduction induced by bone morphogenetic protein

Urist demonstrated that a specific protein purified from bone matrix had osteogenesis, and named it Bone Morphogenetic Protein (BMP). The author employed Urist method to obtain crude BMP, and then implanted it into the mandibular defects of rats. A demineralized bone matrix was obtained from a femur of bovine bone. It was separated and purified by repeated solubilization and precipitation of the protein, and molecular weight of its BMP was evaluated by means of SDS-polyacrylamide gel electrophoresis. BMP was implanted as inlay graft on defect area of rat mandibulae in the experimental groups, and a collagen without BMP was implanted as the same in the preliminary experimental groups, then each group was correlated whether pulsating electromagnetic fields was exposed or not and aperiosteum was preserved or not. Thee histological studies of two groups were employed to observe the osteoinduction process and BMP-induced bone growth on the effect of pulsating electromagnetic fields 1, 2, 3 and 4 weeks after implantation respectively. The results were as follows : 1. The molecular weight of bovine BMP was about 19,100. 2. BMP had a excellent bone forming activity as a implant materials to the repair on the bone defects. 3. The exposure of pulsating electromagnetic fields influenced to the cell differentiation and proliferation since initial stage of ossification as independent from existence of periosteum and BMP, and the exposure of pulsating electromagnetic fields combined with BMP activated the osteoinduction process more. 4. In case of implantation of BMP, preservation with a periosteum showed quicker repair in early ossification stage, but there was no difference between defects and preservation with periosteum after 4 weeks.