DMBA 유도 햄스터 협낭암에서 홍삼 및 TOCOPHEROL의 항암효과에 대한 면역조직화학적 연구

Immunohistochemical study of the inhibitory effect of red ginseng and tocopherol on DMBA induced cancer of the buccal pouch in hamsters

This study was undertaken to observe the effects of red ginseng and tocopherol on 9, 10 - dimethyl - l, 2 - benzanthracene(DMBA) induced cancer of the buccal pouch in hamsters. In this study eighty male golden Syrian hamsters, weighing about 150~200gm, were used and divided into 4 groups: normal group(n=8) - untreated ; control group(n= 24) - DMBA painted ; experimental group I (n=24) - DMBA painted and red ginseng( 15mg/100gm) administered ; experimental group II (n=24) - DMBA painted and tocopherol (2units/100gm) administered. According to groups, the animals were sacrificed every 2 weeks for 15 weeks. Microscopic examination was done and BrdUrd labeling index was calculated after examination of DNA synthetizing cells(S - phase) according to immunohistochemical staining. Distribution of DNA contents of the cell cycle was also measured with flow cytornetry. Comparative results between the various groups are as follows : 1. According to histopathologic features, carcinoma in situ was observed at 11 weeks in the control group and at 13 weeks in the experimantal group I and II. Well differentiated carcinoma was seen at 13 weeks in the control group and at 15 weeks in the experimental group I and II but features in the experimental group I were relatively more severe than those of the experimental group II. 2. In the distribution of the bromodeoxyuridine(BrdUrd) labeled cells, which were compatible to the histological grading, the greatest incorporation of BrdUrd was seen in the cells of the basal layer within the normal epithelium. When dysplatic changes were observed, BrdUrd was more significant in the parabasal cells. However, in carcinoma in situ, BrdUrd showed a disorderly arrangement throughout the whole thickness of the tumor epithelium. 3. BrdUrd labeling index, which correlated with histological findings, has a remarkable significance in nistologic diagnosis of precancerous lesions and prediction of tumor activity. Flow cytometry may be used to evaluate clinical malignancies by detection of aneuploidy and could be used indirectly to predict the period of tumor formation by detecting the distribution of cells in the S - phase and G₂/M phase. 4. Although both red ginseng and tocopherol had inhibitory effects on the DMBA induced cancer of the buccal pouch in hamster, red ginseng seemed to have more inhibitory effect in dysplastic changes and tocopherol seemed to have a more inhibitory effect after expression in carcinoma in situ.