동종골의 탈회에 사용되는 HCL의 S-mutans에 대한 항균 효과

An experimental study of the batericidal effect of the HCL on s-mutans

To determine the bactericidal effect of the HCL, used in demineralization procedure in banked allogeneic bone, we use the modified disk diffusion method using in antibiotic sensitivity tests with S-mutans. Various concentrations of HCL were used to evaluate the pH effect of the HCL on S-mutans from 0.01N to 2.0N. The results obtained were as follows: 1. Minimal bactericidal effects were showed in the 0.5N (pH 2.33). 2. Incresed concentration showed more bactericidal effect on the platting disk. 3. The bactericidal effect showed might be from the lowered pH effect. 4. The vacuum dried disk showed similar bactericidal effects to the HCL exposed disk. We can suppose from the above results that the demineralization procedure with 0.5N HCL may help the sterilization procedure of the allogeneic bone graffs preparation. Freeze-drying procedure after demineralization did not alter the bactericidal effects of HCL. But further study will be needed to evaluate the bactericidal effect of the HCL on non-specific pathogens in oral and maxillofacial regions and the effect on certain virus may be beyond the scope of this experiment.