수종 치과 임프란트의 세포반응에 관한 연구

An in vitro study of the cellular responses to several dental implants

Recently, titanium, hydroxylapatite-coated titanium and nickel titanium have been widely used as dental implants. This study was undertaken to evaluate the cellular responses of mouse fibroblasts which cultured in MEM to the implants. Morphologic changes were observed and cell count and DNA contents were measured on the 1st, 3rd and 7th day. The results obtained from this study were as follows ; 1. The number of the cell was increased accordingly as time at monolayer in all groups including control group, Morphologically, cell process and nucleus became prominent but macroscopic difference to the implants were not observed. 2. As a result of the cell count, there was increased in number of the cell with increasing time. On 1st day, there was no remarkable difference in each group. On 3rd day, control group showed the highest increase of cell number and nickel titanium implant group was higher than other group. On 7th day, increase of cell number was observed in all groups. Titanium implant group showed the highest and nickel titanium implant group were appeared as similar result. 3. In DNA contents measurement, S and G₂M phase cell is less than 20% on 3rd and 7th day. Especially, S and G₂M phase cell was measured 17% and 12% in the titanium and nickel titanium implant group on 1st day. 4. Cell size and DNA content were increased accordingly as time and the highest increase was observed in titanium implant group. These results suggest that all implant materials of titanium that used in this study didn t complicate proliferation of the cultured fibroblasts and are suitable for the clinical use.