인체양막을 이용한 백서 구강점막 결손부에 대한 생물학적 처치

Human amniotic membrane as a biological dressing on the buccal mucosal defects in rats

The purpose of this study was to determine whether use or not human amniotic membrane as a biological dressing for the oral mucosal defects and to compare healing capacity of dry and fresh amniotic membrane. Sixteen rats weighing about 250-300gm were divided into two groups. In group A, the buccal mucosal defect of the left side was allowed secondary epithelization and the right defect was grated with the mucosa taken from the contralateral side. In experimental group B, the left defect was covered with dry amniotic membrane and the right side with fresh amniotic membrane. The animals were sacrificed on the 2nd, 5th, 10th and 20th day after grafting for macroscopic and microscopic examination. The results obtained from this study were as follows : 1. The healing rate of the covering group with amniotic membrane was faster than that of denuded group. 2. The inflammatory response of the covering group with amniotic membrane was less than that of denuded group. 3. In epithelization, the covering group with amniotic membrane was better and faster than denuded group. 4. In inflammatory response and healing process, there were no significant differences between dry and fresh amniotic membrane. 5. The healing rate of the mucosal graft group was faster than that of the others and inflammatory response of the mucosal graft group was a little than that of the others. As above results, it is considered that amniotic membrane is possible to use as biological dressing materials on the oral mucosal defect for wound protection and reepithelization.