Interleukin 2가 DMBA에 유도된 마우스 악하선암에 미치는영향

A study on the effect of Il -2 on DMBA -induced carcinogenesis of the submaxillary blands in mice -distribution of T -cell subsets using monoclonal antibody-

This study was undertaken to evaluate the antitumor effect of IL-2 on the DMBA-induced carcinogenesis at the submaxillary glands of mice. Ninety-five ICR strain 2-3 months old mice, about of weighing 20-30 grams, were divided into a normal group of 3 mice without any treatment, a control 1 group of 32 mice prepared with only incision, a control 2 group of 48 mice treated with DMBA and an experi-mental group of 12 mice prepared with intrasplenic injection when palpable tumor was noted after insertion of DMBA pellets. All salivary glands, regional lymph nodes and spleens were extracted from 95 mice and performed with H-E staining for light microscopic findings and prepared with monoclonal antibodies, that are Anti-Lyt 1, Anti-Lyt 2, for fluorescent microscopic findings. The process of carcinogenesis was observed with light micro-scope and the distribution of T-cell subsets was evaluated with fluorescent microscope. The weight of spleens were measured with electric balance for confirming splenomegaly. The tumor size, that was expressed as the products of the longest perpendicular diameters of lesions, was monitored with Vernier calipers for evaluating the anti-tumor effect of IL-2. Following results were obtained ; 1. Carcinoma in situ was appeared in 8 weeks in control 2 group. 2. Expansion of medullary sinus and aggregation of histiocytes were observed, cancer metastases were not in the regional lymph nodes throughout experimental periods in control 2 group. 3. Macrophages were appeared at 5-week in control 2 group, and hyalinization and splenomegaly were noted in experimental group. 4. The distribution of Lyt-1, Lyt-2 T-cell subsets : There disclosed more Lyt-1 subsets than Lyt-2 subsets generally. ㆍControl 1 group ; It was increased markedly at inflammatory periods (1-3 days), and decreased rapidly and the decreased state was continued throughout the remaining experimental periods. ㆍControl 2 group ; Although some difference was noted among three tissues, the distribution of Lyt-1 subsets increased markedly during the inflammatory periods, and decreased in 2-5 weeks rapidly. It increased again in 8-11 weeks, and decreased from 13th week to 16th week. Lyt-2 subsets decreased more slowly than Lyt-1 subsets. ㆍExperimental group ; Both Lyt-1 and Lyt-2 T-cell were much increased than any other groups. 5. Tumor regression effect of IL-2 ; It was noted that complete response was 16.7%, partial response was 41.7%, minimal response was 25.0%.