BUTYL-CYANOACRYLATE(HISTOACRYLⓇ) 와 FIBRIN SEALANT(TISSEELⓇ)를 이용한 피부이식시 조직치유에 관한 실험적 연구

Experimental study on the application of butyl-cyanoacrylate (histoacrylⓇ) and fibrin sealant (tisseelⓇ) as tissue adhesives in the healing process of skin graft of rats

This study was designed to compare the healing process after skin graft of rats by use of butyl-cyanoacrylate(HistoacryⓇ) and fibrin sealant(TisseeⓇ) as tissue adhesives. In order to carry out the experiment, autogenous skin grafts (about 1㎝ diameter) in left & right abdomen of 60 rats were performed and divided into control & experimental groups. Control group was grafted without tissue adhesives and experimental group was subdivided into two groups which were grafted by the use of butyl-cyanoacrylate(HistoacryⓇ) and Fibrin sealant (TisseeⓇ). Each 4 rats of 60 animals was sacrificed after 1, 3, 5, 7 and 14 days of skin grafts. Hematoxylin-eosin and Masson s trichrome stain were done for the findings of light microscope. The following conclusions could be drawn from the present investigation. 1. In the control groups, there was an aspect of synthesis & replacement of new & mature collagen fibers under skin graft site after 7 days of experiment. 2. In the experimental Ⅰ group, by use of butyl-cyanoacrylate (HistoacryⓇ) under skin graft site, there were the findings of delayed absorption of butyl-cyanoacrylate, remain of inflammatory reaction, and delayed maturation of collagen tissue. 3. In the experimental Ⅱ group by use of fibrin sealant(TisseeⓇ) under skin graft site, it showed that absorbed fibrin sealant and better appearance than experimental Ⅰ group in the consideration of inflammatory reaction & collagen maturation. 4. The maturation and amount of collagen fibers was followed by fibrin sealant, control & butyl-cyanoacrylate group in Masson s trichrome stain. 5. In the case of butyl-cyanoacrylate application, it appears that the use of butyl-cyanoacrylate is better in the purpose of the fixation, because no absorption of butyl-cyanoacrylate under skin graft site results disturbance of vessel anastomosis and healing process. 6. In the case of fibrin sealant application, it indicates that fibrin sealant(TisseeⓇ) has good healing effect because the effect of graft fixation & vessel anastomosis contributes the take of skin graft in the consideration of hemostatic, adhesive and fibrin effect.