백서의 DMBA유도 피부암 발암과정에서의 핵산에 대한 연구

An experimental study on nucleic acid in skin carcinogenesis process of DMBA painted white rats

The purpose of this study was to investigate ability of nucleic acid synthesis in carcinogenesis process. About 80g weighed male White Rats were used in this experimental study, and divided into control and experimental groups. The experimental groups were painted with 0.5% DMBA in mineral oil and the control groups were painted with mineral oil only on the back skin surface two times weekly. The animals were sacrificed at every 1, 3, 5, 7, 9, 11, 13, 15, 17, 19th week and excised the painted area for extraction of nucleic acid. The obtained results were as follows; 1. In carcinogenesis process, changes of DNA amount per g tissue were not remarkable until 9th week, but markedly increased in experimental group at 11th and 13th week. However, there were not much differences after 15th week. Changes of DNA amount per ㎎ protein were not remarkable until 9th week, but it has markedly increased in experimental group after 11th week and continuously increased. 2. In carcinogenesis process, changes of RNA amount per g tissue were not remarkable until 9th week, but markedly increased in experimental group at 11th and 13th week. However, there were not much differences after 15th week. Changes of RNA amount per ㎎ protein were not remarkable until 9th week, but marked increased in experimental group from 11th to 15th week. However, RNA amount per ㎎ protein were decreased after 17th week. From the above, as skin carcinogenesis induced by DMBA, the author suggest that nucleic acid were markedly increased from the detection of tumor by visual inspection and it might be supplied to DNA, RNA for tumor cell divisions. And during tumor progression, nucleic acid were continuously increased and influenced to tumor cell divisions.