技齒創 治療時 Fibronectin의 役割에 관한 免疫細胞化學的 硏究

An immunocytochemical study on the effect of fibronectin on the healing process of extracted wound

The purpose of this study was to search how fibronectin influences to the extracted wound in the process of its normal healing in dogs. This was studied by histopathological, histochemical, and immunocytochemical means. The dogs, weighing average of 10 ㎏, were employed in this experiment. The third and fourth premolars of mandible were extracted under general anesthesia using Ketamine HCl intramuscular injection, and all surgical procedures were performed under aseptic condition. Two dogs of each groups were sacrified on the 1st, 3rd, 5th, 7th, 10th and 14th day respectively after extraction, and specimens were resected. All the specimens were fixed in 10% neutral formalin solution and decalcified with Planko Richlo solution and embedded in paraffin in the usual manner. The 7-8 ㎛ sectioned specimens were stained with hematoxylineosin, Masson s trichrome and avidin-biotin-peroxidase complex (ABC) technique. The results were as follows: The fibronectin was appeared as a granular or reticular pattern on 3rd day, the amount of fibronectin was gradually increased according to the development of granulation tissue but was markedly reduced according the maturation of granulation tissue. Although the fibronectin was markedly increased around the osteoid tissue, it was reduced on calcifying.