DMBA도포가 HAMSTER 협낭상피의 LANGERHANS 세포에 미치는 영향에 관한 실험적 연구

THE EFFECT OF DISTRIBUTICN OF LANGERHANS CELLS IN DMBA-PAINTED HAMSTER CHEEK POUCH EPITHELIUM

The purpose of this study was to investigate the distribution of Langerhans cells on Hamster buccal pouch painted with DMBA (9,10 Dimethyl-1.2-benzanthracene). Golden Hamsters -6week old- were used in this experimental study and devided into control and experimental groups. Experimental groups were painted with 0.5% DMBA in heavy mineral oil on right buccal pouch three times weekly. The animals were sacrificed at every 6. 8. 10. 12. 14. 16th week and excised buccal mucosa for microscopic examination. All specimen stained with Hematoxylin-Eosin and PAP method, and examined by light microscope. The obtained results from this study were as follows. 1. In H-E stain, hyperkeratosis, papillomatous growth, dysplasia, carcinoma in situ, and inflammatory cells were observed in experimental groups. 2. In PAP stain, Langerhans cells were not observed under the submucosa and mucosa of control group and the mucosa of experimental groups. 3. At the early stage of dysplasia in 6th week, Langerhans cells were observed around the dysplasia and markedly increased in 8th week, but slighty decreased in 10 and 12th week. 4. During the formation of dysplasia, Langerhans cells were observed under the submucosa (average no. 5-10), especially aggregated around the blood vessels in cellular dysplastic area. 5. After the formation of carcinoma in situ, Langerhans cells were observed more frequently under the normal submucosa than the peripheral area of cancer tissue and it showed markedly decreasing tendency under the normal submucosa in 16th week and was not observed around the carcinoma in situ.