구내 개방성창상의 초기 치유과정에서의 탄성조직(彈性組織)에 관한 실험적 연구

AN EXPERIMENTAL STUDY ON THE ELASTIC TISSUES IN EARLY INTRAORAL WOUND HEALING

This study was undertaken to demonstrate the time of appearance and the site of origin of elastic fibers in early intraoral wound healing stage, using 12 young rabbits weighing 1.5 ㎏. were anesthesized with seconal given intravenously, open wounds were made on the buccal mucosa to the level of, but not including, the buccinator muscle and on the alveolar mucosa to the level of, but not including, the buccinator muscle and on the alveolar mucosa to the level of, but not including, the periosteum. Normal untreated alveolar and buccal mucosa fiom the contralateral side served as a control. The experimental animals were sacrificed by ether inhalation through every 1, 4, 7, 10, 14, 21 days postoperatively. And the tissues were exicised the buccal and alveolar mucosa including the muscle layer and periosteum, respectively. Specimens were fixed for 48 hours in 10% formalin solution and embedded in paraffin. Serial sections were cut at a thickness of 5 micron, mounted on glass slides and stained using Verhoeff s elastic stain. In light microscopic examinations, following results were obtained; 1. At four days, elastic fibers were observed in the granulation tissue of both alveolar and buccal mucosa in healing stage of intraoral wound of the rabbits. 2. There was a progressive increase in the amount of elastic fibers through the entire time in healing process, but they showed rather decreased in the concentration with fragmentation after 21 days postoperatively in the healing stage of intraoral wound of the rabbits. 3. Elastic fibers were increased in demonstrable concentrations in the perivascular areas and in surrounding the connective tissue and below the muscle layer, subsequently infiltrating the muscle and then appearing in increased numbers at the base of the granulation tissue. Thus, this facts may suspect that the sites of it s origin were smooth muscle and connective tissue.