수종 실험동물 다형핵백혈구의 Candida albicans에 대한 탐식능과 혈청성분과의 유관성에 관한 연구

THE RELATIONSHIP BETWEEN SERUM COMPONENT AND PHAGOCYTIC ACTIVITY TO CANDIDA ALBICANS BY POLYMORPHONUCLEAR LEUKOCYTES IN EXPERIMENTAL ANIMALS AND HUMAN

The author studied the phagocytic and intracellular killing activity between the normal strain and the ultraviolet-ray irradiated strain of Candida albicans by polymorphonuclear leukocytes derived from various laboratory animals, and at the same time, investigated how the serum factors of each laboratory animal influence the above-mentioned activities. The results were as follows; 1. The lethal dose 50 (LD₅₀) of normal Candida albicans in mice were 2.5 × 10⁵ cells/㎖, but could not be observed in the ultraviolet-ray irradiated Candida albicans even with 5 × 10⁷ cells/㎖. It demostrates that the virulence is decreased in the ultraviolet-ray irradiated strains. 2. The phagocytic index of normal strains of Candida albicans by polymorphonuclear leukocytes from laboratory animals and human mixed with homologous sera were 89.2 ± 0.7% in the normal human sera, 56.2 ± 0.5% in the heat-inactivated human sera, and 35.3 ± 0.7% in HBSS. As for rabbits, it was 90.7 ± 0.5% in the normal sera, 67.8 ± 0.8% in the heat-inactivated sera, and 31.2 ± 0.8% in HBSS. As for rats, it was 87.3 ± 0.5% in the normal sera, 65.5 ± 0.7% in the heat-inactivated sera, and 43.3 ± 0.7% in HBSS. And as for mice, it was 93.3 ± 0.8% in the normal sera, 55.2 ± 0.7% in the heat-inactivated sera, and 32.4 ± 0.7 in HBSS. 3. The phagocytic index of ultraviolet-ray irradiated Candida albicans by polymorphonuclear leukocytes from laboratory animals and human mixed with homologous sera were 91.1 ± 0.5% in the normal human sera, 58.3 ± 0.4% in the heat-inactivated human sera, and 27.3 ± 0.5 in HBSS. As for rabbits, it was 96.2 ± 0.7%, 71.3 ± 0.6%, and 26.5 ± 0.7%. As for rats, it was 97.2 ± 0.4%, 64.2 ± 0.9%, and 38.4 ± 0.8%. And as for mice, it was 95.5 ± 0.5%, 55.8 ± 0.2%, and 30.5 ± 0.8%. There was no significant difference between phagocytic effects of the normal strains and the ultraviolet-ray irradiated strains of Candida albicans by polymorphonuclear leukocytes, however, the phagocytic effects were decreased when the sera were heat-inactivated or when HBSS were added in place of the sera. 4. No significant difference in the phagocytic effects was observed between the homologous sera and heterologous sera. 5. The intracellular killing index of Candida albicans by poly-morphonuclear leukocytes mixed with the normal homologous sera from human and laboratory animals showed marked decrease in comparison with the phagocytic index. The intracellular killing index was 52.4 ± 0.9% with the rat PMN cells, 43.7 ± 0.2% with the rabbit PMN cells, 32.4 ± 0.7% with the human PMN cells, and 23.6 ± 0.1% with the mouse PMN cells. 6. The complement titers of the sera from the laboratory animals and human by CH₅₀ were 28.1 unit in rat sera, 21.4 unit in rabbit sera, 23.1 unit in rat sera, and 1.7 unit in mouse sera. 7. The germ tube formation activity of Candida albicans in various sera from laboratory animals showed marked differences between the normal strains and the ultraviolet-ray irradiated strains. It was markedly higher in the normal strains. The highest was 91.5% in the normal strains treated with the rabbit sera, and the lowest was 40.7% in the normal strains treated with the rat sera. 8. The clumping phenomena could be observed both in the normal sera and in the heat-inactivated sera, however, it showed a decreasing tendency in the immunized sera. It was also observed that the germ tube production increased with the increase of clumping phenomena, and at the same time, they showed some specific materials adhered to the cell surface when the clumping increased, but none such materials were seen when the clumping decreased.