인체 다형핵백혈구에 의한 Candida albicans 탐식작용시 혈청성분 분포에 관한 연구

LOCALIZATION OF SERUM COMPONENTS ON CANDIDA ALBICANS FOR PHAGOCYTOSIS BY HUMAN POLYMORPHONUCLEAR LEUKOCYTE

Opsonins, IgG and complement components C3, are known to aid in the phagocytosis of albicans. In this study, the author investigated ability of phagocytosis between normal human serum and heat inactivated serum by use of the phagocytic index. The author observed electron-microscopically, the binding site of IgG and C3 in an attempt to find the opsonical differences between normal serum and heat inactivated serum. The results were summarized as follows: 1. Phagocytic activities of Polymorphonuclear leukocyte (PMN) were compared to C. albicans, normal human serum was most active (89.5±0.9%), and heat inactivated serum(57.2±0.9%) were least active.HBSS (34.8±1.2%) were found to be poor in phagocytosis. 2. The ultrastructural changes were observed electron-microscopically. The cell wall of C. albicans which was engulfed by PMN became thicker, and electron dense materials like azurophile and specific granules were deposited. 3. When C. albicans were treated by Peroxidase-labeled antihuman IgG, there was a high density of IgG on the cell wall and fibrillic structure of C. albicans in normal human serum. But lower density of IgG were observed in the heat inactivated serum. Under HBSS, there were no IgG on the cell wall of C. albicans. 4. Although a high density of C3 was deposited on the fibrillic structures of the cell wall of C. albicans which were treated by peroxidase-labeled antihuman C3 in normal human serum, there were deposited no C3 in the heat inactivated serum.