가토 과립구에 의한 Candida albicans 탐식작용시 수종의 효소분비에 대한 전자현미경적 연구

ELECTRON MICROSCOPIC STUDIES ON THE RELEASE OF ENZYMES FROM RABBIT GRANULOCYTES IN PHAGOCYTOSIS OF CANDIDA ALBICANS

The cytoplasmic granules of granulocytes contains a variety of antimicrobial substances which are directly responsible for the inactivation and hydrolytic enzymes which play a role in digestion and killing of invaded microorganisms. In this study, the author investigated phagocytic index of Candida albicans by granulocytes, and determined enzyme activity changes of acid phosphatase and alkaline phosphatase, β-glucuronidase before and after phagocytosis, and observed localization of enzyme activity by electron microscope. 1. The phagocytic index of Candida albicans by granulocytes was 93.7 ± 0.7%. 2. Alkaline phosphatase activity was 0.18 ± 0.17 μM in granulocyte itself and 5.46 ± 1.18 μM after phagocytosis. 3. Acid phosphatase activity was 4.83 ± 0.47 μM in granulocyte itself and 12.57 ± 2.73 μM after phagocytosis, and β-glucuronidase was 1.0 ± 0.7 ㎍ and 4.0 ± 0.5 ㎍. 4. In 30 minutes after phagocytosis, electron microscopic findings revealed that the electron density of azurphil granule-like materials aggregated adjacent to the Candida albicans. As a late event it revealed that electron density of azurphil granule-like materials deposited at the surroundings of Candida albicans. 5. In 2 hours after phagocytosis, electron microscopic findings revealed that alkaline phosphatase activity was localized between the ingested Candida albicans and phagocytic vacuole, and acid phosphatase and β-glucuronidase activity were localized at the surroundings of Candida albicans, membrane structure and endoplasmic reticulum.