ALLOXAN유도 당뇨백서의 혈청과 과립구가 CANDIDA ALBICANS에 미치는 영향에 관한 연구

A study on the effects of the serum and the granulocytes of the alloxanized diabetic rats on candida albicans

In the present study, investigations were undertaken whether the growth of Candida albicans is influenced by glucose, altered the serum factors and the granulocytes in diabetes mellitus. The rats were grouped into the normal, alloxan-diabetic, insulin-treated groups and bred with the commercial diets for 8 days. In the normal and insulin-treated groups, the body weights were gained 26 gm and 18 gm respectively, but in the alloxan-diabetic groups, the weights were lost 15 gm. The plasma glucose concentrations in the normal, alloxan-diabetic and insulin-treated groups were 153 ± 5 ㎎%, 386 ± 7㎎%, 114 ± 2㎎%. The growth rates of Candida albicans in the alloxan-diabetic groups were 1.18 ± 0.01 (1hr), 1.36 ± 0.02 (2hrs), 1.67 ± 0.03 (3hrs), 2.01 ± 0.07 (4hrs). These results were significantly higher than those of the normal groups (p ＜ 0.05). In insulin-treated groups, the growth rates of Candida albicans were 1.13 ± 0.01 (1hr), 1.25 ± 0.01 (2hrs), 1.44 ± 0.01 (3hrs), 1.46 ± 0.01 (4hrs). These were significantly lower than those of the alloxan-diabetic groups (p ＜ 0.05). The growth rates of C. albicans showed no significant difference between the normal and the insulin-treated groups. In the high glucose groups (400 ㎎%), the growth rates showed little difference with the normal, alloxan-treated, insulin-treated groups. In the control groups which had no added serum, the growth rates were much higher than those of the other experimental groups. The actual doubling time calculated from the growth curves obtained from the experimental groups revealed that the control groups without added serum (3.52 ± 0.07 hrs) were the shortest of all diet groups and alloxan-diabetic groups were the next. These results suggest that the growth rates of Candida albicans were influenced by some factors in serum and these factors might be decreased or impaired in the diabetic animals and through the administration of the insulin, these factors seemed to be recovered in serum. Also the glucose alone might not be the contributing factor for Candidal growth. The pagocytic capacities of the granulocytes showed no difference between the experimental groups. However, the killing capacities of the granulocytes from alloxan-diabetic groups were significantly lower as compared to the normal and insulin-treated group (p ＜ 0.05). The specific activities of the myeloperoxidase were the same in the normal, alloxan-diabetic and insulin-treated groups. However, the protein volume were significantly lower in the alloxan-treated groups as compared to the normal and insulin-treated groups (p ＜ 0.05). These results suggest that in diabetes mellitus, the phagocytic capacities are not altered but the intracellular killing capacities are decreased. Myeloperoxidase, one of the intracellular defense enzymes, was not changed in diabetes mellitus, but the protein volume in diabetic granulocyte which affectes on the myeloperoxidase concentration were decreased because of the lack of insulin in the alloxan-diabetic rats which leades to the generalized decrease in the intracellular killing activity of the granulocytes.