cis-Platinum이 구강암 Hamster의 협낭 및 타액선에 미치는 영향에 관한 실험적 연구

An experimental study on the effects of cis-Platinum on the buccal pouch and the salivary gland in the hamster with oral cancer

This study was undertaken to observe the effects of cis-Platinum on the buccal pouch and parotid gland in the hamster with DMBA-induced oral carcinoma. 36 golden Syrian hamsters-7 week of age, about 100 gram of body weight-were used in this study. Eighteen hamsters, induced epidermoid carcinoma in the buccal pouch by painting 0.5% DMBA 3 times a week for 12 weeks served as experimental group and another eighteen sound hamsters as control group. Both the fifteen experimental & control group were injected intraperitoneally 50㎎ of cisplatinum per ㎡ of body surface with single dose respectively. The hamsters were sacrificed before administration and 1st, 2nd, 3rd, 4th, 5th week after administration of cis-platinum. The buccal pouch and parotid gland were excised and processed in a routine manner for Hematoxylin-Eosin and periodic acid Shiff stain. After microscopic observation following results were obtained 1. The buccal pouches & parotid glands of control group revealed normal histologic findings without periodic changes. 2. In experimental group, the neoplastic region on buccal pouch in first week showed micro scopically more reduced proliferative findings than before administration of cis-Platinum, but epithelial dysplasia and proliferation were gradually developed from the 2nd week. 3. Damage on the parotid gland tissues of experimental group before administration of cis-Platinum may be construed due to application on the excessive area with DMBA, diffusion of the inflammation and degeneration of tissue by tumor. 4. In the experimental group, the parotid glands began to recover from 1st week after administration of cis-Platinum and showed normal histological findings in the 2nd week, but remained partial acinic cell proliferation until the 5th week. 5. In PAS reaction, the buccal pouches and parotid glands of control group showed normal reaction without periodic change. 6. In PAS reaction, epithelial layer of the buccal pouch associated with carcinoma in the experimental group revealed irregular reaction; more malignant, distinct negative. Fibrosis region in the stromal tissue showed strong positive reaction. 7. In PAS reaction, the acini of the parotid glands in the experimental group before administration of cis-Platinum showed irregular reaction, but began to recover from 1st week and returned to normal reaction from 3rd week after the administration, whereas the basement membrane of acini and ductal epithelium showed normal reaction without periodic change.