당뇨백서 악하선에서의 Na⁺, K⁺ activated adenosine triphosphatase에 관한 실험적 연구

An experimental study on Na⁺, K⁺ activated adenosine triphosphatase in submaxillary gland of alloxanized rat

Effect of insulin deficiency and insulin replacement therapy on body weight, the weight of submaxillary gland and Na⁺, K⁺ adenosine triphosphatase (Na⁺, K⁺ ATPase) in submaxillary gland of albino rats were observed. Experimental study of the Na⁺, K⁺ ATPase of submaxillary gland was done by a deoxycholate-treated procedure and the results were obtained as follow ; 1. Alloxan treated group revealed marked decrease of the body weight and weight of submaxillary gland than control group. These changes were recovered by insulin replacement therapy. 2. Blood glucose level of control group was 93.5 ± 2.74㎎%, alloxan group was 230.3 ± 16.05 ㎎%, and insulin treated group was 110.6 ± 3.7 ㎎% respectively. 3. Result of double treatment of sodium deoxycholate on the tissue of submaxillary gland was presented higher (9.3%) specific activity of Na⁺ K⁺ ATPase than single treatment of sodium deoxycholate, but specific activity of Mg⁺⁺ ATPase was not influenced by the times of treatment of sodium deoxycholate. 4. The specific activity of Na⁺, K⁺ ATPase of alloxanized rats was lowered about 20% than control group. These change were recovered by insulin replacement therapy. These results suggested that the lower specific activity of Na⁺, K⁺ ATPase and loss of weight of submaxillary gland had close effects on salivary secretion.