성견악하선에서 Na⁺, K⁺-activated Adenosine Triphosphatase에 대한 실험적 연구

AN EXPERIMENTAL STUDY ON NA⁺, K⁺-ACTIVATED ADENOSINE TRIPHOSPHATASE IN DOG SUBMAXILLARY GLAND

Experimental study of purificating trials and specific activity of Na⁺, K⁺-activated Adenosine Triphosphatase in dog submaxillary gland was done and results were obtained as follow; 1. In homogenate, the specific activity of Na⁺, K⁺-ATPase was 0.63 μmoles/min/㎎ㆍprotein. 2. In microsomal fraction, the specific activity of Na⁺, K⁺-ATPase was 2.79 μmoles/min/㎎ㆍprotein. The enzyme was purified 4.4-fold than in homogenate and recovery rate was 80%. 3. In discontinuous gradient, the specific activity of Na⁺, K⁺-ATPase was 20.41 μmoles/min/㎎ㆍprotein. The enzyme was purified 32.4-fold than in homogenate and recovery rate was 20%. 4. In linear gradient, the specific activity of Na⁺, K⁺-ATPase was the highest at fraction 11, and the density was 1.10~1.12g/㎖ at that. The specific activity was 36.29 μmoles/min/㎎ㆍprotein, and the enzyme was purified 57.6-fold than in homogenate.