抗癌化學療法劑가 白鼠唾液腺組織에 미치는 影響에 關한 實驗的 硏究

EXPERIMENTAL STUDY ON THE EFFECTS OF ANTINEOPLASTIC ON THE SALIVARY GLAND IN THE WHITE RAT

This study was undertaken to observe the salivary gland of white rat when administrated to single dose of antineoplastic agents: 5-Fluorouracil, Cyclophosphamide, and Methotrexate. Eighty eight Sprague Dawley strain white rats, weighing about 180 grams were used and devided into control and three experimental groups. Sixteen rats were injected 0.5cc of normal saline solution intravenously as placebo in control group. In experimental groups, each twenty four rats in group I, II and Ill were injected intravenously 200mg. 5-Fluorouracil, 32mg. Cyclophosphamide, and 2.5mg. Methotrexate per kilogram body weight with single dose respectively. Rats were serially sacrificed on 1st, 3rd, 5th, 7th, 10th, 14th, 21st, 28th day after injection of placebo and antineoplastic agents. At sacrifice, the parotid, submandibular, and sublingual gland were dissected out in toto, and stained with 1) Hematoxylin and Eosin: 2) Periodic Acid Shiff. After examination and comparision of all specimen, the results of this study were obtained as follow. 1. The severity of tissue damage after injection of antineoplastic agents showed duct and acinar, whereas serous and mucous gland in order. 2. Parotid gland showed rather high cytotoxic effect while submandibular and sublingual gland showed varied cytotoxic deffect which is thought due to a mixed gland. 3. The cytotoxic effects showed Cyclophosphamide, 5-Fluorouracil, and methotrexate in order. 4. The most destructive salivary gland was on 3rd day after injection of antineoplastic agent and began to repair after 7th day. 5. In PAS reaction, severe change was observed especially in serous gland while irregularity of reaction revealed.