數種 化合物이 培養 骨端 軟骨 蛋白質 生合成에 미치는 影響에 關한 硏究

EFFECTS OF SOME COMPOUND ON THE BIOSYNTHESIS OF PROTEINS IN RAT EPIPHYSEAL CARTILAGE IN VITRO

The epiphyseal cartilage tissues of one day old Sprague Dawley rat tibia were incubated in Krebs-glucose medium contained ³H-proline with or without some effecting compound. At the end of incubation the whole cartilage tissues were homogenized. The homogenates and culture medium were treated with 0.1% sodium dodecyl sulfate (SDS) and 0.1% beta-mercaptoethenol. After dialysis, the ³H-labeled samples were counted in a scintillation counter. The ³H-hydroxyproline content of some samples was measured by the method of Juva and Prockop(l966). The separation of the protein fractions of some samples was performed with SDS-polyacrylamide disc gel electro-phoresis. The results obtained were as follows; 1. The total incorporation of ³H-proline were reduced in the presence of all ex-perimental compound. 2. The increase of concentration of hot water extract of Artemisia asiatica in the culture medium caused reduction in the incorporation of ³H-proline into epiphyseal cartilage tissues and secreted proteins. 3. When the epiphyseal cartilage tissues were incubated with concentration of diterpenoid compound D of Siegesbeckia pubescens in the range of 0.125mg/ml to 0.50mg/ml, the total incorporation of praline was progressively inhibited, and the hydroxylation of ³H-proline was not the dose dependence. 4. By the SDS-polyacrylamide gel electrophoregram of the cultured epiphyseal cartilage tissue proteins, three major peaks corresponding to a-, β-chain and albumin were observed in control samples. But peaks in same positions of the gel were also o~served in the presence of diterpenoid compound D, althougl the distribution of radioactivity was more heterogenous.