數種 化合物이 培養 骨端 軟骨 蛋白質 生合成에 미치는 影響에 關한 硏究
EFFECTS OF SOME COMPOUND ON THE BIOSYNTHESIS OF PROTEINS IN RAT EPIPHYSEAL CARTILAGE IN VITRO
- 대한구강악안면외과학회
- 대한구강악안면외과학회지
- 대한구강악안면외과학회지 제5권 제1호
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1979.1231 - 40 (10 pages)
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The epiphyseal cartilage tissues of one day old Sprague Dawley rat tibia were incubated in Krebs-glucose medium contained ³H-proline with or without some effecting compound. At the end of incubation the whole cartilage tissues were homogenized. The homogenates and culture medium were treated with 0.1% sodium dodecyl sulfate (SDS) and 0.1% beta-mercaptoethenol. After dialysis, the ³H-labeled samples were counted in a scintillation counter. The ³H-hydroxyproline content of some samples was measured by the method of Juva and Prockop(l966). The separation of the protein fractions of some samples was performed with SDS-polyacrylamide disc gel electro-phoresis. The results obtained were as follows; 1. The total incorporation of ³H-proline were reduced in the presence of all ex-perimental compound. 2. The increase of concentration of hot water extract of Artemisia asiatica in the culture medium caused reduction in the incorporation of ³H-proline into epiphyseal cartilage tissues and secreted proteins. 3. When the epiphyseal cartilage tissues were incubated with concentration of diterpenoid compound D of Siegesbeckia pubescens in the range of 0.125mg/ml to 0.50mg/ml, the total incorporation of praline was progressively inhibited, and the hydroxylation of ³H-proline was not the dose dependence. 4. By the SDS-polyacrylamide gel electrophoregram of the cultured epiphyseal cartilage tissue proteins, three major peaks corresponding to a-, β-chain and albumin were observed in control samples. But peaks in same positions of the gel were also o~served in the presence of diterpenoid compound D, althougl the distribution of radioactivity was more heterogenous.
I. 緖論
II. 實驗材料 및 方法
III. 實驗結果
IV. 考察
V. 結論
REFERENCES
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