白鼠肝壞死 및 肝硬變症과 唾液腺病變과의 相關關係에 關한 實驗的 硏究

A STUDY OF SALIVARY GLAND IN RELATION WITH EXPERIMENTALLY INDUCED LIVER NECROSIS AND CIRRHOSIS OF ALBINO RATS

In order to observe the salivary gland in relation with liver necrosis and cirrhosis, 98 male albino rats of 3 to 4 months old, weighing 150 to 190gm, were selected and divided into two groups. 1. Control group: 14 rats were fed on a ready-made diet and sacrifiecd at intervals of 1, 3, 5, 5, 7, 14 and 28 days, the liver and the salivary gland were extracted. 2. Experimental group: 84 rats were fed on the same diet as the control group. At intervals of every 3 days, a single dosage(0.1cc/100gm bodyweight) of CC1₄, were injected into the peritoneal cavity. The experimental groups are further subdivided on the basis of duration of injections. a. Group 1(21 rats): total number of injections two times in the period of 6 days. b. Group 2(21 rats): total number of injections four times in the period of 12 days. c. Group 3(21 rats): total number of injections six limes in the period of 18 days. d. Group 4(21 rats): total number of injections eight times in the period of 24 days. Each experimental group was then sacrificed at the intervals of 1, 3, 5, 7, 14 and 28 days. As in the control group, the salivary gland and the liver were extracted, at the time of sacrifice without resort to anesthesia. The extracted glands and liver were immediately fixed in 10% neutral Formaline solution for 24 hrs. Via paraffin embedding, the tissuse were prepared in 4-5μ in thickness. H & E. stain was used in the liver tissue whereas in the preparation of the salivary gland PAS & Alcian blue were used in additon to H & E stain. The results of this study were as follows: 1) In histopathologic study of the liver, it was concluded that in the group 1, only the mild necrosis was observed. The group 2, moderate necrosis was observed but no evidence of the fibrotic changes were seen. However, in group 3, severe necrosis was seen and fibrotic changes were partially seen in the 28th day sacrificed animals. The group 4 resulted in the liver cirrhosis. 2) Very little differences if any on the salivary glands of group with mild and moderate liver necrosis when compared to that of the control group. 3) In the salivary gland of severe necrosis group, the swelling in the regoin of acini and the hydrophic degeneration in the epithelial cells of the striated duct were observed. In the duct of submaxillary gland infiltrations of inflammatory cells were noticed. PAS and Alcian blue reaction were observed to be more decreased in comparison to the control group. 4) The hydrophic changes in the acini and the epithelial cells of the striated ducts were more severe in the group 4 when compared to the group 3. Further PAS and Alcian blue reaction were observed to be more decreased in the group 4, in comparison to the group 3.