디젤엔진 排氣物質의 變異原性 硏究

Mutagenicity of Diesel Exhaust Emission by Micronucleus Test

Interest in the studies of diesel e앙laust errùssion has been increasing by the expected increase in the use of diesel powered automobiles out of concideration of fuel economy. It was well known that diesel exhaust ernission was mutagenic in the bioassay as Ames test. The authors tried to find out the cytogenetic effect of diesel exhaust ernission by the operational condition of engine such as speed and load. For the investigation of those effects, 66 male rnice of ICR strain were used. The benzene-ethanol extracts of diesel exhaust ernission were injected intra peritoneum 25mg/kg and 50mg/kg respectively. To evaluate the cytogenetic effect, mouse bone rnarrow rnicronucleus test was carried out. The frequency of rnicronucleus was different among the various groups according to the operational conditions of engine. The frequency of rnicronucleus ìn idling group was the highest of all the groups ; the subgroup of 50mg/kg showed the rate of 1.30%, 25mg/kg subgroup 0.55%. And the group of 2000rpm with 50% load showed the lowest rate of rnicronucleus appearance as 0.20% and 0.15%. In general, the frequency of rnicronucleus was shown higher in propotion to load and was shown inversely proportional to speed.