카드융독성을 명가하기 위한 방법으로서의 염색체이상 및 자매염색체 교환

Chromosome Aberration and Sister Chromatid Exchange for the Assessment of Cadmium Toxicity

This study was performed to investigate the applicability of 9 chromosome aberration and sister chromatid exch없ge analysis for the assessment of cytotoxicity and cytogenetic effects of cadnuum lnduction of chron losome aberration and sister chromatid exchange in CHO-Kl cells and hurnan peripherallymphocytes by 2 hour-treatment of CdCIz with various concentrations was observed in relation to their frequencies and types of aberration. The frequency of chromosome aberration in CHO cells treated with CdC1 2 at G1 was increased with dose-dependent manner. When human peripheral lymph。이tes were treated with cadnùum at G 0 and harvested at 72 hours there after, the response was dose-dependent and all the aberrations were also chromatid types. There was no significant increase in frequencies of sister chromatid exchange in both CHO cells and human lymphocytes treated with different concentrations of cadnùum. It was suggested that SCE analysis was not a good assessment method for cadmium toxicity.