銀Ion 및 역상(逆相) HPLC를 이용(利用)한 입체이성체적(立體異性體的) 분석(分析)에 의한 은행종실유(銀杏種實油)의 트리글리세리드의 분자종(分子種)에 관한 연구(硏究)

The Stereospecific Analysis of the Triacylglycerols of Ginkgo Nut Oils by High-Performace Liquid Chromatography(HPLC) in the Silver Ion and Reversed Phase Modes

Triacylglycerols of the seeds of Ginkgo biloba have been resolved by high-performace liquid chromatography(HPLC} in the silver-ion and reverse-phase modes. The fatty acids were identified by a combination of capillary gas chromatography and gas-chromatography /mass spectrometry as the methyl and /or picolinyl ester. The main components are $C_{18:2{\omega}6}$ 수식 이미지(39.0mol%), $C_{18:1{\omega}7}$ 수식 이미지(asclepic acid 21.5mol%), and $C_{18:1{\omega}9}$ 수식 이미지(oleic acid, 13.8mol%). Considerable amounts of unusual acid such as $C_{20:3{\Delta}^{5,11,14}$ 수식 이미지 (5.7mol%), $C_{18:2{\Delta}^{5,9}$ 수식 이미지(2.8mol%), and $C_{18:3}{\Delta}^{5,9,12}$ 수식 이미지(1.6mol%), were checked. In addition, an anteiso-branched fatty acid, 14-methylhexadecanoic acid, was also present as a minor component(0.9 mol%). The triacylglycerols were separated into 17 fractions by reverse-phase HPLC, and the fractionation was achieved according to the partition numnber(PN) in which a ${\Delta}^5$ 수식 이미지-non methylene interrupted double bond($^5$ 수식 이미지-NMDB) showed different behaviour from a methylene interrupted double bond in a molecule with a given cahinlength. Silver-ion HPLC exhibited excellent resolution in which fractions(23 fractions) were resolved on the basis of the number and configuration of double bonds. In this instance, the strength of interaction of a ${\Delta}^5$ 수식 이미지-NMDB system with silver ions seemed to be weaker than a methylene interrupted double bond system. The principal triacylglycerol species are as follows ; $(C_{18:2{\omega}6)2}/C_{18:1{\omega}7}$ 수식 이미지, $C_{18:1{\omega}9}/C_{18:1{\omega}7}/C_{18:2{\omega}6}$ 수식 이미지, $(C_{18:1{\omega}7)2}/C_{18:2{\omega}6}$ 수식 이미지, $C_{16:1{\omega}7}/C_{18:1{\omega}9}/C_{20:3}{\Delta}^{5,11,14}$ 수식 이미지, $C_{16:1{\omega}7}/C_{18:1{\omega}7}/C_{20:3}{\Delta}^{5,11,14}$ 수식 이미지, $C_{18:1{\omega}9}/C_{18:1{\omega}7}/C_{18:2{\omega}6}$ 수식 이미지, $C_{18:1{\omega}9}/C_{18:2}{\Delta}^{5,5}/C_{20:3}{\Delta}^{5,11,14}$ 수식 이미지, $(C_{18:1{\omega}7)2}/C_{18:2{\omega}6}$ 수식 이미지 and $(C_{18:1{\omega}9)2}/C_{18:2{\omega}6}$ 수식 이미지, while simple triacylglycerols without $C_{18:2{\omega}6})_3$ 수식 이미지 were not present. Stereospecific analysis showed that fatty acids with ${\Delta}^5$ 수식 이미지-NMDB system and saturated chains were predominantly located at the site of sn-3 carbon of glycerol backbones. It is evident that there is asymmetry in the distribution of fatty acids in the TG molecules of Ginkgo nut oils.