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Baker s Yeast로부터 1,4-Benzoquinone Reductase의 분리

Isolation of 1,4-Benzoquinone Reductase from Baker s Yeast

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An intracellular, soluble 1,4-benzoquinone reductase was purified from Baker s Yeast by ammonium sulfate precipitation, DEAE-Sephacel anion exchange chromatography, and Sephacryl S-200 gel filtration chromatography. 1,4-Benzoquinone reductase was achieved 123.8 fold purification from crude homogenate with a yield of 11.1%.

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