역상(逆相)-HPLC와 $Ag^+$ 수식 이미지-HPLC에 의한 잣기름의 트리아실글리세롤분자종(分子種)의 상호분리(相互分離)

Studies on Resolution of the Molecular Species of Triacyl-glycerols in the Seed of Pinus koraiensis by HPLC in the Reverse-phase and Ag-ion Modes.

The lipids from the seeds of Pinus koraiensis mostly composed of triacylglycerols (TGs), in which linoleic acid (46.2 mol%) and oleic acid (25.6 mol%) are present as main components in the fatty acid composition. Surprisingly, they also have unusual fatty acids with ${\Delta}^5$ 수식 이미지-double bond systems such as ${\Delta}^{5.9.12}-C_{18:3}$ 수식 이미지 (16.0 mol%), ${\Delta}^{5.9}-C_{18:2}$ 수식 이미지 (2.3 mol%) and ${\Delta}^{5.11.14}-C_{20:3}$ 수식 이미지 (0.8 mol%). Saturated fatty acids of palmitic, stearic and arachidic acid were present in less than 8.0 mol%. TG was resolved into 17 fractions by reverse-phase HPLC according to so-called partition number (PN) suggested by Plattner, in which TG molecules with ${\Delta}^{5}$ 수식 이미지-NMDB acyl chains eluted later than did those with ${\Delta}^{9}$ 수식 이미지-MDB acyl radicals. $Ag^+$ 수식 이미지-HPLC separated the TG into 14 fractions more clearly than did those with ${\Delta}^{9}$ 수식 이미지-MDB acyl radicals. $Ag^+$ 수식 이미지-HPLC separated the TG into 14 fractions more clearly than did reverse-phase HPLC, and the complexity of ${\Delta}^{5.9.12}-C_{18:3}$ 수식 이미지 moiety with silver ion impregnated in the column bed was in the level between ${\Delta}^{9.12.15}-C_{18:3}$ 수식 이미지 ($C_{18:3{\omega}3}$ 수식 이미지) and $C_{18:2{\omega}6}$ 수식 이미지 (${\Delta}^{9.12}-C_{18:2}$ 수식 이미지). In the $Ag^+$ 수식 이미지-HPLC, it was found that the molecular species having a given-numbered double bonds widely spreaded in the acyl chains eluted earlier than those concentrated in one acyl chain. The main molecular species are $(C_{18:2{\omega}6})_2/{\Delta}^{5.9.12}-C_{18:3}$ 수식 이미지 (14.8 mol%), $C_{18:1{\omega}9}/C_{18:2{\omega}6})_2$ 수식 이미지 (12.8 mol%) and $C_{18:1{\omega}9}/C_{18:2{\omega}6}/{\Delta}^{5.9.12}-C_{18:3}$ 수식 이미지 (10.9 mol%).