입본특이적(立本特異的) 방법(方法)과 $^{13}C-NMR$ 수식 이미지 기법(技法)에 의한 잣기름의 트리아실 글리세롤의 구성지방산(構成脂肪酸)의 분포(分布)에 관한 연구(硏究)

Studies on the Fatty Acid Distribution in the Position of Triacylglycerols from the Seed of Pinus Koraiensis by Stereo-specific Analysis and $^{13}C-NMR$ 수식 이미지 Techniques

All the triacylglycerols including the molecular species having ${\Delta}^5$ 수식 이미지-unsaturated fatty acids from the seeds of Pinus Koraiensis, were split into a mixture of diacylglycerols by a Grignard reagent prepared with allyl bromide without arousing acyl chains of a glycerol moiety to migration, and were also easily partially hydrolyzed to diacylglycerols by pancreatic lipase. (S)-(+)-(1-naphthyl)ethyl urethane(NEU) derivatives of the diacylglycerol mixture derived from the triacylglycerols were fractionated into sn-1, 3-, sn-1, 2- and sn-2, 3-DG-NEU by silica-HPLC and the fatty acid composition of these fractions was analysed. $C_{18:1{\omega}9}$ 수식 이미지 is distributed evenly in the three positions of TG with $C_{18:2{\omega}6}$ 수식 이미지 mainly located in sn-2 position, while ${\Delta}^5$ 수식 이미지-unsaturated fatty acids such as ${\Delta}^{5.9}-C_{18:2}$ 수식 이미지, ${\Delta}^{5.9.12}-C_{18:3}$ 수식 이미지 and ${\Delta}^{5.11.14}-C_{20:3}$ 수식 이미지 are exclusively present in the sn-3 position. These results could be confirmed by $^{13}C$ 수식 이미지-NMR spectroscopy : the signals at $^{\delta}$ 수식 이미지173.231 ppm and $^{\delta}$ 수식 이미지172.811 ppm of the carbonyl carbon of acyl moieties indicate the presence of saturated acids and/or $C_{18:1{\omega}9}$ 수식 이미지 (oleic acid) in the ${\alpha}({\alpha}- or ${\beta}$ 수식 이미지- positions, and $C_{18:2{\omega}6}$ 수식 이미지 including $C_{18:1{\omega}9}$ 수식 이미지 in the ${\beta}$ 수식 이미지-position, respectively. In addition, the resonance at $^{\delta}$ 수식 이미지173.044 ppm suggested a location of ${\Delta}^5$ 수식 이미지-unsaturated fatty acid moiety in the ${\alpha}({\alpha}-position.