메틸글리코시드에 의한 지방산의 효소적 배당화

Enzymatic Glycosylation of Fatty Acids by Methyl Glycosides

Glycoside fatty acid esters were synthesized by lipase-catalyzed glycosylation of fatty acids with methyl glycoside in solvent and solvent free process. Optimum condition of solvent process using 2-methyl-2-propanol were : moral ratio of methyl glycoside to fatty acid 1:3: initial concentration of methyl glycoside 50g/l:enzyme(immodilized lipase Novozym 435 from Candidia antarctica) content 1%(w/v) : desiccant content 9%(w/v); reaction temperature $60^{\circ}C$ 수식 이미지: reaction time 10hrs. The yield of 99% was obtained. Solvent-free process was carried out in total absence of solvent at $70^{\circ}C$ 수식 이미지 under reduced pressure, 5-20mmHg. To give meximum yield of 99% at the optimum condition of molar ratio of methyl glycoside to fatty acid 1:3, enzyme content 10%(w/w), and reaction time 10hrs. The glycosylation reactivity of different glycosylation agents were sequent to $Methyl-{\beta}-D-fructofuranoside$ 수식 이미지. $Methyl-{\beta}-D-glucopyranoside$ 수식 이미지. $Methyl-{\beta}-D-fructofuranosi$ 수식 이미지 de, and $Methyl-{\alpha}-D-glucopyranoside$ 수식 이미지.