逆相-HPLC와 Ag+ -HPLC에 의한 잣기름의 트리아실글리세롤 分子種의 相互分離

Studies on Resolution of the Molecular Species of Triacyl-glycerols in the Seed of Pinus koraiensis by HPLC in the Reverse-phase and Ag-ion Modes

The lipids from the seeds of Pinus koraiensis mostly composed of triacylglycerols (TGs), in which linoleic acid (46.2 mol%) and oleic acid (25.6 mol%) are present as main components in the fatty acid composition. Surprisingly, they also have unusual fatty acids with △^5-double bond systems such as △^5.9.12-C_18:3 (16.0 mol%), △^5.9-C_18:2 (2.3 mol%) and △^5.11.14-C_20:3 (0.8 mol%). Saturated fatty acids of palmitic, stearic and arachidic acid were present in less than 8.0 mol%. TG was resolved into 17 fractions by reverse-phase HPLC according to so-called partition number (PN) suggested by Plattner, in which TG molecules with △^5-NMDB acyl chains eluted later than did those with △^9-MDB acyl radicals. Ag^+-HPLC separated the TG into 14 fractions more clearly than did those with △^9-MDB acyl radicals. Ag^+-HPLC separated the TG into 14 fractions more clearly than did reverse-phase HPLC, and the complexity of △^5.9.12-C_18:3 moiety with silver ion impregnated in the colum bed was in the level between △^9.12.15-C_18:3 moiety with silver ion impregnated in the column bed was in the level between △^9.12.15-C_18:3 (C_18:3ω3) and C_18:2ω6 (△^9.12-C_18:2). In the Ag^+-HPLC, it was found that the molecular species having a given-numbered double bonds widely spreaded in the acyl chains eluted earlier than those concentrated in one acyl chain. The main molecular species are (C_18:2ω6)_2/△^5.9.12-C_18:3 (14.8 mol%), C_18ω9/(C_18:2ω6)_2 (12.8 mol%) and C_18:1ω9/C_18:2ω6/△^5.9.12-C_18:3 (10.9 mol%).