芸香科(운향과) 植物 種實의 抗酸化性 物質에 관한 硏究

Studies on the Antioxidative Substances in the Seeds of the Rutaceae Family

Some seeds of the Rutaceae family, Zanthoxylum piperitum, Z. schinifolium, Evodia officinalis, Poncirus trifoliata, Citrus unshiu, were investigated to clarify their antioxidative components. Finely powdered samples were extracted by hexane. followed by dichloromethane and then 70% methanol in a hot bath. Its unsaponifiables containing α-and γ-tocopherol with trace amount of β-and δ-tocopherol, also showed comparatively weak activity, although the hexane fraction itself had no significant antioxidative effect on lard. Levels of total tocopherols in the samples averages 42. 24-154. 11 mg/l00g total extractives. The dichloromethane-and 70% methanol extractives showed strong antioxidative activity, from which antioxidative substances were purified with benzene-acetone(6:5, V/V) on a silica gel column, and with a solvent mixture of acetonitrile-methanol-H₂O(40:40:20, V/V/V) on a Sep-Pak C₁₈ hydrolyzed by 5% KOH-ethanol. The recovered unsaponifiables were, then, separated on a colunm of high performance liquid chromatogrqphy. The unsaponifiables produced by hydrolysis of the isolates from dichloromethane extractives has epi-catechin(40.0~57.l%) and (+)-catechin(19.1~24.4% to total phenolic substances, on area base) as major component, accompanied by chlorogenic acid, gallic acid(?), trans-p-coumaric acid and trans-p-ferulic acid including some unknown components, and those derived from 70% methanol extractives also comprise (+)-catechin(31.3~39.6% to total components, on area base), epi-catechin(20.2~36.4%), trans-p-coumaric acid(8.4~15.3%) and trans-p-ferulic acid(7.7~14.1%) as predominant component with some minor coponents, but the fraction supposed to be gallic acid (?) is not present. The antioxidative activities of the phenolic components isolated in this work were in order of epi-catechin＞catechin＞chlorogenic acid＞trans-p-ferluic acid＞trans-p-coumaric acid.