동물성 수산식품 중의 Sterol 조성과 Cholesterol Δ₇-dehydrogenase의 존재에 관한 연구

Studies on the Composition of Sterol and the Presence of Cholesterol Δ₇-dehydrogenase in Marine Animal Products

Levels of sterols including Δ₇-dehydrocholesterol isolated from the tissues of marine animal products (20 species) were determined on 1.5% OV-17 column of gas-liquid chromatography. The composition showed that the mussels and clams contained various sterols in their tissues : cholesterol, brassicasterol, 24-methylenecholesterol with some minor components such as 22-trans-norcholesta-5,22-dien-3β-ol, 22-cis-dehydrocholesterol, 22-trans-dehydrocholesterol, desmosterol. 7-dehydrocholesterol, campesterol, stigmasterol, β-sitosterol, isofucosterol, and 7-dehydrocholesterol which could be converted into vitamin D₃in the skin tissue of animal was present in the muscle of oyster, Crassostrea gigas. On the other hand, the others including gastropoda were predominantly composed of cholesterol. The minor sterols such as 24-methylenecholesterol, stigmasterol and β-sitosterol in the fish intestines are supposed to be derived from dietary plankton. Cholesterol Δ₇-dehydrogenase which could convert cholesterol into Δ₇-dehydrocholesterol was present in the pickles of Tricurus haumela intestine.