새싹 추출물의 항산화 작용과 미량 성분

In this study, the anti-oxidative effects and components of sprout serum were investigated. In the buds, high levels of zinc, iron and manganese were analyzed in addition to copper, magnesium, and potassium. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity of sprout serum was evaluated with IC₅₀. IC₅₀ of sprout serum was 58.0 mgmL⁻¹, and that of vitamin C was 3.7 mgmL⁻¹. In the test of superoxide scavenging activity of sprout serum, the activity was dependent on the concentration of serum. In this case, the IC₅₀ was 2.0 wt%. Cell viability was detected by using the MTT method. Cultured human fibroblast was treated with 15 mM H₂O₂ and cell viability was 70% in case of control. However, the effect of treating 0.5% of sprout serum was similar to that of 0.0001% of vitamin C.