곤달비추출물의 항산화와 멜라닌 생성 저해 효과

Effect of Antioxidation and Inhibition of Melanogenesis from Ligularia stenocephala extract

In this study, we evaluated anti-oxidation and whitening effects of Ligularia stenocephala extract for use as the cosmeceuticals. L. stenocephala was extracted by three different solvents which was n-Hexane, ethyl acetate, H₂O. The free radical (1,l-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity of extract of L. stenocephala was in the order: ethyl acetate fraction of leaf (IC₅₀ value of 10.512ug/mL) 〉 ethyl acetate fraction of stem (IC₅₀ value of 31.877ug/mL) 〉 H₂O fraction of leaf (IC₅₀ value of 129.194ug/mL). Reactive oxygen species (ROS) scavenging activity of extract of L. stenocephala was in the order: ethyl acetate fraction of leaf (IC₅₀ value of 0.230mg/mL) 〉 ethyl acetate fraction of stem (IC₅₀ value of 0.528mg/mL) 〉 H₂O fraction of leaf (IC₅₀ value of 0.799mg/mL). Tyrosinase inhibition activity of L. stenocephala extracts was reduced 29.477% on ethyl acetate fraction of leaf, 13.583% on ethyl acetate fraction of stems. Therefore, L. stenocephala extracts may be useful as a new antioxidant and whitening agent to inhibit melanogenesis.