연두금파리(Lucilia illustris) 유충 혈림프의 용균효소(Lysozyme)

Identification and Characterization of Lysozyme in Haemolymph of the Larvae of Lucilia illustris

Lysozyme is one component of the humoral defence system of the insects. Lysozyme in haemolymph of injured larvae of Lucilia illustris was identified and characterized with acidic electrophoresis and inhibition zone assay. Lytic activity against cell walls of Gram positive Bacteria shows simillar effect in Bacillus megateriam and Micrococcus luteus. Lysozyme activity from injured haemolymph was higher than that of naive haemolymph. this enzyme was comparatively stable for pH and heat treatment, but heat stability was decreased drastically over 60°C. Lysozyme has been purified from injured haemolymph by reverse-phased FPLC.