누리장나무 추출물의 세포독성 및 항산화 활성효과 연구

A Study on Cytotoxicity and Antioxidant Activity of Clerodendrum Trichotomum Extract

In this study, an antioxidant activity, anti-inflammatory and antibacterial experiment was conducted to develop natural cosmetic materials depending on biological activity and cytotoxicity of the Clerodendrum trichotomum extract and the results were as follows. The content of polyphenol was 119.723±1.0㎎/g and when different concentrations of the extract were applied, DPPH radical scavenging activity was measured at 3.2, 4.0, 13.5, 24.7, 77.1, and 78.1% respectively. This means that the higher the concentration, the higher antioxidant activity. For antibacterial measurement, S. aureus, S. epidermidis, E. coli, and P. acnes were added. As a result, S. aureus and P. acnes had the highest value(16㎜) at a concentration of 1㎎/㎖, S. aureus had the highest value(16㎜) at a concentration of 5㎎/㎖, and S. epidermidis had the highest value(15㎜) at a concentration of 10㎎/㎖. Also, S. aureus and S. epidermidis had the highest value(16㎜) at a concentration of 20㎎/㎖. ABTS radical scavenging activity was measured at 0.645, 4.677, 15.054, 31.774, 101.237, and 101.022% respectively, when different concentrations of the extract were applied. The result was greater than or similar to Vit and C, which are standard controls, at a concentration of 500 and 1000㎍/㎖. The cell survival rate was 92.738, 84.260, 83.964, 86.987, 86.589, and 70.497% and there was no cytotoxicity of the extract. The expression of iNOS declined when th concentration of the extract was 500㎍/㎖ and in the COX-2 experiment, RAW 264.7 using the pre-treated extract failed to inhibit COX-2 that increased due to LPS. In conclusion, it is considered that the findings of this study can be used as important basic data to develop natural cosmetic materials using the Clerodendrum trichotomum extract.