대하의 부위별 지질과 지방산 대사율

Lipid and Fatty Acid Metabolic Rate in the Different Portions of Oriental Shrimp

This study was undertaken to investigate the experimental basis on the lipid metabolism of the marine under-evolved animal from the view point of comparative biochemistry. Oriental shrimp, penaeus orientalis, was taken as a crustacea sample and the process of fatty acid biosynthesis and the bioconversion of eicosapentaenoic acid(EPA) were investigated to track out the metabolic pathway of the fatty acid in the animals of a lower order by using isotope marked tracers, acetate-1-¹⁴C and eicosapentaenoate-1--¹⁴C. The results were summarized as follows : A Biosynthesis of fatty acids from acetate ; Distribution of radioactivity was measured at 12 and 24 hr after injecting the isotope marked tracers, acetate-1-¹⁴C, into the survived oriental shrimp and the followings were resulted in the experiment on fatty acid biosynthesis from acetate. 1. About 70% of the radioactivity was detected in hepatopancreas and only about 2% in gonad. 2. In polar and non-polar lipid, 16:0, 18:0, 16:1 and 18:1 acid have been found to be biosynthesized from acetate, while 18:2n-6, 20:2n-6, 20:5n-3, 22: 1 and 22:6n-3 were not to be biosynthesized from it. Consequently, about 90% of the radioactivity has been detected in saturated and monoenoic acids. B. Bioconversion of isotope marked eicosapentaenoate, EPA-1-¹⁴C; Specific activity of fatty acids of total lipid extracted from the each portions of body has been measured at 5, 24, and 72 hour after the injection of EPA-1-¹⁴C. The results were summarized as follows. 1. The specific activity was the highest in hepatopancreas and the lowest in gonad. 2. It was assured thar it is impossible to bioconvert EPA to any others in the all organs of oriental shrimp, since most of radioactivity was detected in EPA extracted from the portions of the body and fractionated.