여수연안 및 동중국해의 세균상: 3. 분리균중 protease 생산균의 동정 및 효소생산조건

Bacterial Flora of East China Sea and Yosu Costal Sea Areas: 3. Identification of the Protease Producing Bacteria and the Producing Conditions for the Enzyme in the strain isolated

During 6th~14th August 1992, 23 strains having protease activity were isolated from East China sea. Two strains(F₄-188 and F₄-193) that protease activity is the most strong were seleted by second screen. Results investigated for enzyme producing condiction with identification of these two strains were as follow: F₄-188 and F₄-193 having the strong protease activity were classified with pseudomonas sp. and protease activity 65.5 unit/㎖, 74.6 unit/㎖, respectively. The optimum pH for enzyme activity was alkaline protease with pH 8.0 in F₄-188 strain and acid protease with pH 6.0 in F₄-193 strain. The highest protease activity for nitrogen source and carbon source was ammonium sulfate(1.7%) and starch(2.1%) in F₄-188 strain, albumin(1.5%) and α-cellulose(1.0%) in F₄-193 strain, respectivly. Effect of metal salt on the enzyme production in F₄-188 and F₄-193 strains was calcium chloride and magnecium sulfate, respectively. The optimum temperature for growth of two strains was 30℃ and heat-resisting property enzyme not to effect in 60 minutes at 40℃ but more that 90% were disappeared with heat treatment for 10 minutes at 80℃.