살조개, Protothaca jedoensis의 성성숙 유도 및 산란 유발

Spawning Induction and Gonadal Maturation Induction of the Venus Clam, Protothaca jedoensis

In order to obtain the basic information for artificial seedling production of venus clam, Prototlzaca jedoensis, gonadal maturation was induced by different temperature, and fertilization and hatching rate were investigated by various stimulating methods for spawning induction. The gonadal maturation induction was evaluated by percentage of follicle and oocyte area on the ovary of the female through the histological observation of gonadal tissue at 18℃ and 22℃. The percentage of follicle area was the maximum value (96.38±1.36%) in 16 May and oocyte area was 74.67±3.67% at 22℃. Serotonin injection stimulus could induce the spawning in venus clam. Water temperature rising, air dry, pulse spawning(gonad incision), sperm suspension solution, UV irradiated seawater, and NH₄OH, H₂O₂addition were not effective. Serotonin injection and temperature rising were responsed 70%, 100%, respectively. The response time to initial spawning in the serotonin injection stimulus(10⁻⁴M) was within 3 minutes on female and 2 minutes on male. The highest fertilization and hatching rate was 95.5%, 87.2%, respectively.