방사무늬김 색소변이형 사상체 수용액의 흡수스펙트럼

Comparative study on absorption spectra of water extracts of the sporothallus of pigmentation mutants in prophyra yezoensis udea(bangiales, rhodophyta)

Comparative study was made on absorption spectra (300-750 ㎜) of water extracts centrifuged (5,000 rpm, 5min.) of the sporothallus of the wild-type (U-511) plant, and the red-type (H-25), the green-type (C-O giant), the yellow-type (ZY-1), the violet-type (ZV-1), the gray-type (ZGr-1), the light red-type (ZLR-1), the light green-type (C-31) and the light yellow-type (ZLY-1) mutants in P. yezoensis. The absorption was recorded with a double monochrometer recording spectrophotometer (Hitachi U-3210) not equipped with an integrating sphere attachment or a head-on photomultiplier. The wild-type plant and the red-, the green-, the violet- and the light red-type mutants showed the spectra having a maximum in near ultraviolet region (300-400 ㎜), indicating the presence of the ultraviolet absorbing substance. The wild-type plant and the red-, the green-, the yellow- and the violet-type mutants showed the spectra having two absorption maxima in 530-580 ㎜ region. The yellow- and the gray-type mutants had the spectra with markedly low absorption in a 300-750 ㎜ region. The violet- and the gray-type mutants were characterized by higher PE/Chl. a and PC/Chl. a ratios. The light green-type mutant was marked by lower PE/Chl. a and PE/PC ratios, while the light yellow-type by higher PE/Chl. a and PE/PC ratios. The light red-type mutant was featured by higher PE/Chl. a and PE/PC ratios and by lower PC/Chl. a ratio. It is inferred : that the light green- and the light yellow-type mutants might result from qualitative and quantitative variations of phycoerythrin ; that the gray- and the light red-type mutants might originate from qualitative variation of phycoerythrin and quantitative variation of chlorophyll a and phycocyanin; that the violet-type mutant might arise from quantitative variation of chlorophyll a; that the yellow-type might stem from quantitative variation of phycocyanin.