HPLC에 의한 원지의 생리활성 성분 분석법 개발 및 함량 모니터링

Quantitative Analysis and monitoring of Bioactive Marker Compounds from Polygalae Radix by High Performance Liquid Chromatography.

Quantification monitoring of 74 samples of Polygalae Radix collected from Korean and China was conducted following the analysis method from Chinese Pharmacopoeia (CP) 2015 edition. However, this method is considered not accuracy enough because tenuifolin was chosen to be the only marker component for the quality control of Polygalae Radix. The de-sugar products of onjisaponins and oligosaccharide esters including tenuifolin(1), 3,4,5-trimethoxycinnamic acid(2), 4-methoxy cinnamic acid methyl ester(3) were isolated from the alkaloid hydrolysis products of extracts from Polygalae Radix. A novel simultaneous quantification of the three components by HPLC-UV was developed, and quantification mon-itoring for 17 samples collected from China and Korea was conducted. The results from the new analysis method improved complicated preprocessing method and presented the standard as the using of grand contents.