Facility for high resolution cryo-electron microscopy of biological macromolecules at Korea Basic Science Institute

Cryo-electron microscopy (cryo-EM) single particle analysis has become a mainstream structural biology tool and therefore access to high-end transmission electron microscope (TEM) dedicated to high resolution cryo-EM is critical. At Korea Basic Science Institute (KBSI), a facility dedicated to biological electron microscopy was established in 2016, and High Resolution Bio-TEM (HR Bio-TEM, Titan Krios, Thermo Fisher Scientific Inc., USA) is now accessible to national and international researchers. This report describes major features of the HR Bio-TEM and the facility, along with overall workflow of cryo-EM image data acquisition.