Streptomyces sp. L77-140 이 생산하는 항생물질의 분리정제 및 동정
Purification and Identification of an Antibiotic Produced from Streptomyces sp. L77-140
- 한국산업기술융합학회(구. 산업기술교육훈련학회)
- 산업기술연구논문지
- 산업기술교육훈련논문지 제16권 4호
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2011.1269 - 75 (7 pages)
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In the course of the screening for microorganism producing antibiotics, Streptomyces sp. L77-140 was discovered which produced a water-soluble basic antibiotic L77-140. By using paper disk anti-microbial assay, the productivity of antibiotic from Streptomyces sp. L77-140 was tested by several carbon and nitrogen sources such as soluble starch and soybean flour, respectively. L77-140 showed anti-microbial activities against Gram-positive and Gram-negative bacteria. From the culture supernatant, L77-140 was purified by cation exchange chromatography and silicagel column chromatography. In the pH stability test, L77-140 was stable under acidic and alkali conditions. Based on the spectroscopic measurement including Ultra Violet(UV), In Frared(IR), Nuclear Magnetic Resonance (NMR) and mass spectrum(Mass) analyses, L77-140 was identified as spenolimycin.
Ⅰ. 서 론
Ⅱ. 실험 방법
Ⅲ. 실험결과 및 고찰
Ⅳ. 결 론
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