고초균 유래 2-디옥시리보오스 5-포스페이트 알돌라아제 유전자(deoC )의 클로닝, 발현 및 특성연구
Cloning, Expression and Characterization of 2-Deoxyribose Phosphate Aldolase Gene(deoC) from Bacillus sp.168
- 한국산업기술융합학회(구. 산업기술교육훈련학회)
- 산업기술연구논문지
- 산업기술교육훈련논문지 제18권 3호
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2013.0999 - 103 (5 pages)
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A deoC gene encoding 2-Deoxyribose phosphate aldolase(DERA) was cloned from Bacillus subtilis 168 into an expression vector and protein was expressed in Escherichia coli. A molecular weight of the expressed recombinant DRRA was approximately 24.5kDa. The recombinant DRRA was purified using Ni-NTF affinity chromatography and then characterized. The degree of purification yield of the purified recombinant DERA were 1.4-fold and 74%, respectively, compared with that of the crude extract. The optimum temperature and pH of the enzyme were 20∼50℃ and pH6.0, respectively. The thermostability of the enzyme showed that more than 42% activity remained after at 40℃ for 24h, but no activity over 60℃ at the same condition.
Ⅰ. 서 론
Ⅱ. 실험재료 및 방법
Ⅲ. 실험결과 및 고찰
Ⅳ. 결 론
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