재조합 효모균의 회분배양 최적화의 생산성 향상

Enhanced Production of Peroxidase(PA1) and Culture Optimization of Recombinant Saccharomyces Cerevisiae

The study on enhanced production of peroxidase(POD) and culture optimization of recombinant S. cerevisiae GPD-PODl was investigated with 5L jar fermentor. The peak of extracellular POD activity was detected at 20 h. The secrered yield of POD activity was found to be 87%. The maxirnal and stable POD activity was obtained with yeast nitrogen base w/o amino acid 6.7 g/L at 30℃, pH 8. The POD activity was increased up to 73-fold in a flask cultivation and enhanced by more than 155-fold in a laboratorical scale fermentor cultivation. The resultant maximum POD activity was obtained with respect to the control process of the respiratory quotient.(RQ) Additional improvement is likely to be obtained through a refinement of biotic control strategies.