Streptomyces sp. L79-333 배양액 유래의 항균활성물질의 분리정제

Purification of Antibiotic from Culture Broth of Streptomyces sp. L79-333

The strain designated as Streptomyces sp. L79-333 produced a water-insoluble compound, named as KP-2, which showed anti-microbial activities against several Gram-positive and negative bacteria under paper disk anti-microbial assay. The production of KP-2 in Streptomyces sp. culture broth was exarnined using several carbon and nitrogeo sources such as maltose and skim milk for their effects on growth of Streptomyces sp. as well on antibiotic activity. KP-2 was purified from the culture supernatant by 2-step silica gel column chromatography followed by solvent extraction. KP-2 represented a high pH stability under acidic and alkaline conditions showing more than 80%. Based on the studies of its spectroscopic properties using ultraviolet(UV) and infrared(IR), and fast atomic bombardment mass(FAB-Mass) spectrum analyses, KP-2 was identified as echinomycin. Echinomycin, also known as quinomycin A, is ao quinoxaline group antibiotic.