SSR 분자마커를 이용한 색소 및 비색소옥수수 핵심집단의 유전분석

Genetic Analysis of Core Sets of Colored Maize and Non-Colored Maize Inbred Lines using SSR Markers

In this study, genetic diversity, genetic relationship, population structure and association analysis among 12 colored and non-colored maize lines were investigated using 300 simple sequence repeat (SSR) markers. In genetic diversity analysis, a total of 1,331 alleles were identified at all the loci with an average of 4.44 and a range between 2 and 10 alleles per locus. The major allele frequency varied from 0.25 to 0.92 with an average of 0.48. Among 1,331 alleles, 221 alleles were colored maize specific allele, and 408 alleles were non-colored maize specific allele. While 702 alleles were shared in colored and non-colored maize lines. In addition, colored maize specific alleles were detected in 163 SSR markers. From population structure analysis, the 12 colored and non-colored maize lines were separated with based on the membership probability threshold 0.8, and divided into groups I, II, III and admixed group. The results of this study could serve as useful molecular markers for identification and characterization of colored and non-colored maize lines in the maize breeding studies.